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alphalisa assay kits  (Revvity)


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    Revvity alphalisa assay kits
    Alphalisa Assay Kits, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alphalisa+insulin+kit/Insulin/bio_rxiv__2023__08__01__551291-166-0-14
    Average 91 stars, based on 263 article reviews
    alphalisa assay kits - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Incubation:

    Article Title: Discovery of a novel series of indolinylpyrimidine-based GPR119 agonists: Elimination of ether-a-go-go-related gene liability using a hydrogen bond acceptor-focused approach
    Article Snippet: .. After incubation, supernatants from each well were collected, and secreted insulin concentration was measured using AlphaLISA insulin kit (Perkin Elmer) according to the manufacture’s instruction. ..

    Article Title: Discovery of a novel series of GPR119 agonists: Design, synthesis, and biological evaluation of N-(Piperidin-4-yl)-N-(trifluoromethyl)pyrimidin-4-amine derivatives
    Article Snippet: .. After incubation, supernatants from each well were collected, and secreted insulin concentration was measured using AlphaLISA insulin kit (Perkin Elmer) according to the manufacture’s instruction. ..

    Concentration Assay:

    Article Title: Discovery of a novel series of indolinylpyrimidine-based GPR119 agonists: Elimination of ether-a-go-go-related gene liability using a hydrogen bond acceptor-focused approach
    Article Snippet: .. After incubation, supernatants from each well were collected, and secreted insulin concentration was measured using AlphaLISA insulin kit (Perkin Elmer) according to the manufacture’s instruction. ..

    Article Title: Discovery of a novel series of GPR119 agonists: Design, synthesis, and biological evaluation of N-(Piperidin-4-yl)-N-(trifluoromethyl)pyrimidin-4-amine derivatives
    Article Snippet: .. After incubation, supernatants from each well were collected, and secreted insulin concentration was measured using AlphaLISA insulin kit (Perkin Elmer) according to the manufacture’s instruction. ..

    Isolation:

    Article Title: Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis
    Article Snippet: .. Insulin released from isolated islets or monolayer cells was measured by an AlphaLISA insulin kit (PerkinElmer) or insulin high range and ultra-sensitive kits (Cisbio). ..

    other:

    Article Title: Berberine is an insulin secretagogue targeting the KCNH6 potassium channel
    Article Snippet: Insulin secretion was measured using an AlphaLISA insulin kit with an EnVision 2101 multilabel reader (PerkinElmer Life Sciences, San Jose, CA, USA).



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    Fig. 1 | Optimization of a CRISPR screen for insulin content in EndoC-βH1. a, Pipeline for a genome-wide CRISPR LoF screen in EndoC-βH1 from viral transduction (left) to antibiotic selection (middle) and a final FACS selection followed by sequencing and enrichment analysis of integrated sgRNAs (right). b,c, FACS staining for intracellular insulin in INS-silenced EndoC-βH1 (siINS, blue) or their respective non-targeting controls (siNT, pink) as individual plots (b) or histogram overlay (c). d,e, The associated mRNA expression of INS (d) and corresponding levels of intracellular insulin as measured by <t>alphaLISA</t> (e). f,g, FACS staining using the insulin antibody (blue) or isotype control (pink) in the human embryonic kidney cell line HEK293T as individual plots (f) or histogram overlay (g). h, Pipeline for a small-scale CRISPR screen in EndoC-βH1 targeting only three genes (NMS, INS and PAM) alongside EV control cells. FACS sorting gates for low insulin (dashed line) were determined based on control cells
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    Fig. 1 | Optimization of a CRISPR screen for insulin content in EndoC-βH1. a, Pipeline for a genome-wide CRISPR LoF screen in EndoC-βH1 from viral transduction (left) to antibiotic selection (middle) and a final FACS selection followed by sequencing and enrichment analysis of integrated sgRNAs (right). b,c, FACS staining for intracellular insulin in INS-silenced EndoC-βH1 (siINS, blue) or their respective non-targeting controls (siNT, pink) as individual plots (b) or histogram overlay (c). d,e, The associated mRNA expression of INS (d) and corresponding levels of intracellular insulin as measured by <t>alphaLISA</t> (e). f,g, FACS staining using the insulin antibody (blue) or isotype control (pink) in the human embryonic kidney cell line HEK293T as individual plots (f) or histogram overlay (g). h, Pipeline for a small-scale CRISPR screen in EndoC-βH1 targeting only three genes (NMS, INS and PAM) alongside EV control cells. FACS sorting gates for low insulin (dashed line) were determined based on control cells
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    Fig. 1 | Optimization of a CRISPR screen for insulin content in EndoC-βH1. a, Pipeline for a genome-wide CRISPR LoF screen in EndoC-βH1 from viral transduction (left) to antibiotic selection (middle) and a final FACS selection followed by sequencing and enrichment analysis of integrated sgRNAs (right). b,c, FACS staining for intracellular insulin in INS-silenced EndoC-βH1 (siINS, blue) or their respective non-targeting controls (siNT, pink) as individual plots (b) or histogram overlay (c). d,e, The associated mRNA expression of INS (d) and corresponding levels of intracellular insulin as measured by <t>alphaLISA</t> (e). f,g, FACS staining using the insulin antibody (blue) or isotype control (pink) in the human embryonic kidney cell line HEK293T as individual plots (f) or histogram overlay (g). h, Pipeline for a small-scale CRISPR screen in EndoC-βH1 targeting only three genes (NMS, INS and PAM) alongside EV control cells. FACS sorting gates for low insulin (dashed line) were determined based on control cells
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    Image Search Results


    Fig. 1 | Optimization of a CRISPR screen for insulin content in EndoC-βH1. a, Pipeline for a genome-wide CRISPR LoF screen in EndoC-βH1 from viral transduction (left) to antibiotic selection (middle) and a final FACS selection followed by sequencing and enrichment analysis of integrated sgRNAs (right). b,c, FACS staining for intracellular insulin in INS-silenced EndoC-βH1 (siINS, blue) or their respective non-targeting controls (siNT, pink) as individual plots (b) or histogram overlay (c). d,e, The associated mRNA expression of INS (d) and corresponding levels of intracellular insulin as measured by alphaLISA (e). f,g, FACS staining using the insulin antibody (blue) or isotype control (pink) in the human embryonic kidney cell line HEK293T as individual plots (f) or histogram overlay (g). h, Pipeline for a small-scale CRISPR screen in EndoC-βH1 targeting only three genes (NMS, INS and PAM) alongside EV control cells. FACS sorting gates for low insulin (dashed line) were determined based on control cells

    Journal: Nature genetics

    Article Title: A genome-wide CRISPR screen identifies CALCOCO2 as a regulator of beta cell function influencing type 2 diabetes risk.

    doi: 10.1038/s41588-022-01261-2

    Figure Lengend Snippet: Fig. 1 | Optimization of a CRISPR screen for insulin content in EndoC-βH1. a, Pipeline for a genome-wide CRISPR LoF screen in EndoC-βH1 from viral transduction (left) to antibiotic selection (middle) and a final FACS selection followed by sequencing and enrichment analysis of integrated sgRNAs (right). b,c, FACS staining for intracellular insulin in INS-silenced EndoC-βH1 (siINS, blue) or their respective non-targeting controls (siNT, pink) as individual plots (b) or histogram overlay (c). d,e, The associated mRNA expression of INS (d) and corresponding levels of intracellular insulin as measured by alphaLISA (e). f,g, FACS staining using the insulin antibody (blue) or isotype control (pink) in the human embryonic kidney cell line HEK293T as individual plots (f) or histogram overlay (g). h, Pipeline for a small-scale CRISPR screen in EndoC-βH1 targeting only three genes (NMS, INS and PAM) alongside EV control cells. FACS sorting gates for low insulin (dashed line) were determined based on control cells

    Article Snippet: Insulin was quantified using the Insulin (human) AlphaLISA Detection kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content, respectively.

    Techniques: CRISPR, Genome Wide, Transduction, Selection, Sequencing, Staining, Expressing, Control